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Degrees
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M.Sc. Ph.D.
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My Expert Service
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I have been a researcher for last 13 years with extensive experience in retroviral biochemistry, retroviral enzymology, retroviral replication, drug discovery and protein-nucleic acid interactions. My scientific expertise is substantiated by an impressive publication record with more than twenty original articles published in international journals of high repute. My prominence in the field is authenticated by the fact that my work has been widely disseminated and acknowledged by scientific community with more than 300 citations in the scientific literature. Throughout my career, my research has been selected to be presented in the form of poster/oral presentation at National and International Meetings along side that of the experts from academia and industry in more than three dozen times. For my excellence in research, I have been awarded several highly prestigious award including the National Institutes of Health Pathway to Independence Award, Fellows Award for Research Excellence by the National Institutes of Health, etc. In addition, my research has featured in several publications, newspaper articles and websites. For my contribution to science, I have been elected to be member of prestigious scientific societies such as the American Society for Biochemistry and Molecular Biology, Sigma-Xi, American Chemical Society, etc. I have served as ad-hoc reviewer for several journals and have co-chaired national and international conferences on various occasions.
Currently, I am an assistant professor at the Center for AIDS Health Disparities Research at Meharry Medical College, which is part of part of the Vanderbilt-Meharry Center for AIDS Research at Nashville, TN. My field of research is retroviral replication with special emphasis on HIV. My laboratory utilizes chemical biology, molecular virology, biochemistry, and structural biology techniques to probe replication complexes in HIV to gain insight in HIV replication. We anticipate that understanding the replication complexes will pave way for new targets for drug discovery against HIV/AIDS. In addition, I have also experience in teaching microbiology, biochemistry, chemistry, molecular genetics, etc.
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Experience & Qualifications
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Work Experience:
09/09-Current: Assistant Professor Center for AIDS Health Disparities Research, Meharry Medical College
Nashville, TN
•Evaluating HIV-1 integrase inhibitors as potential drugs for HIV/AIDS.
•Examining role of genome structure and organization on HIV-1 integration and replication.
•Examining role of nucleoside analogs as inhibitors of HIV-1 Reverse transcriptase RNase H function.
•Evaluating nanoparticles as agents for prevention of HIV-1 infection.
•Evaluating nanoparticles as drug delivery vehicles for HIV/AIDS.
04/02-09/09: Research Fellow
HIV Drug Resistance Program, National Cancer Institute-NIH, Frederick, MD.
•Cloned, expressed and purified the insoluble reverse transcriptase from Bordotella pertusis.
•Utilised pyrrolo-deoxycytidine analog to investigate structural anomaly in HIV-1 polypurine tract containing DNA/RNA hybrid.
•Used Locked Nucleic Acids (LNA)/Abasic analogs to decipher the role of nucleic acid structure on retroviral and retrotransposon reverse transcription.
•Developed LNA-based Fluorescent In Situ Hybridization assay to detect microsatellite RNA in embryonic stem cells.
•Deciphered the role of unstructured DNA template on the polymerase function of HIV-1 reverse transcriptase.
07/96-04/02: Junior and Senior Research Fellow:
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India. (Supervisor: Dr. Mala Rao)
•Purified and biochemically characterized a peptidic aspartic protease inhibitor (ATBI) from an extremophilic Bacillus sp.
•Studied the kinetics of inhibition and structural interaction of ATBI with HIV-1 protease and other related aspartic proteases.
•Demonstrated the presence of a molten globule like intermediate in the folding pathway of HIV-1 protease.
•Studied the electrostatic interactions of aspartic proteases with lipid membranes and colloidal gold particles.
Technical Skills
•Protein Biochemistry: Expression, Purification, Western Blotting, Isoelectric Focusing, Inhibition mechanisms, Rate constant determination, etc.
•Molecular Biology Techniques: Cloning and Expression of Recombinant Proteins, Gene Expression in Cell Free Cellular Component System, PCR Techniques Hybridization Techniques, Co-Expression of Proteins, Dual Expression Cassette Design, DNA/RNA Synthesis Techniques.
•Biophysical studies: Fluorescence, CD, Chemical Modification of Active Site Residues, Anisotropy.
•Protein-Nucleic acid Interactions: Gel mobility shift assay, Chemical foot printing.
•Molecular Virology: Transfection, Virus titer, Virus Infection, Luciferase Assay, Real Time PCR, FACS analysis.
Memberships in Professional & Honorary Societies:
•American Society for Biochemistry and Molecular Biology (ASBMB)
•Sigma-Xi
•Center for Cancer Research Fellows and Young Investigators (CCRFYI)
•Virology Interest Group at the National Institutes of Health
Professional Experience in Leadership Positions:
Committee Member Distinguished Seminar Series at NCI-Frederick.
•Co-Chaired the Molecular and Cellular Biology Session of CCRFYI Retreat 2004 and 2005.
•Committee Member CCRFYI in 2004.
•Committee Member for the Outstanding Mentor Award at the NCI.
Advisor: Guidepoint Global Advisors
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Available Modes Of Communication
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email/chat
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Contact
CV Dash
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