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Degrees
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M.Sc. Ph.D.
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My Expert Service
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Teaching Expertise:
Biochemistry, Microbiology, Virology, Molecular Genetics, Molecular Biology, Biotechnonolgy
Writing Expertise:
1.Scientific manuscript writing/reviewing.
2. Grant writing/reviewing.
Scientific Expertise:
NIH funded Researcher with 13 years of experience in conducting research in retrovirology.
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Experience & Qualifications
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Work Experience:
09/09-Current: Assistant Professor Center for AIDS Health Disparities Research, Meharry Medical College
Nashville, TN
•Evaluating HIV-1 integrase inhibitors as potential drugs for HIV/AIDS.
•Examining role of genome structure and organization on HIV-1 integration and replication.
•Examining role of nucleoside analogs as inhibitors of HIV-1 Reverse transcriptase RNase H function.
•Evaluating nanoparticles as agents for prevention of HIV-1 infection.
•Evaluating nanoparticles as drug delivery vehicles for HIV/AIDS.
04/02-09/09: Research Fellow
HIV Drug Resistance Program, National Cancer Institute-NIH, Frederick, MD.
•Cloned, expressed and purified the insoluble reverse transcriptase from Bordotella pertusis.
•Utilised pyrrolo-deoxycytidine analog to investigate structural anomaly in HIV-1 polypurine tract containing DNA/RNA hybrid.
•Used Locked Nucleic Acids (LNA)/Abasic analogs to decipher the role of nucleic acid structure on retroviral and retrotransposon reverse transcription.
•Developed LNA-based Fluorescent In Situ Hybridization assay to detect microsatellite RNA in embryonic stem cells.
•Deciphered the role of unstructured DNA template on the polymerase function of HIV-1 reverse transcriptase.
07/96-04/02: Junior and Senior Research Fellow:
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India. (Supervisor: Dr. Mala Rao)
•Purified and biochemically characterized a peptidic aspartic protease inhibitor (ATBI) from an extremophilic Bacillus sp.
•Studied the kinetics of inhibition and structural interaction of ATBI with HIV-1 protease and other related aspartic proteases.
•Demonstrated the presence of a molten globule like intermediate in the folding pathway of HIV-1 protease.
•Studied the electrostatic interactions of aspartic proteases with lipid membranes and colloidal gold particles.
Technical Skills
•Protein Biochemistry: Expression, Purification, Western Blotting, Isoelectric Focusing, Inhibition mechanisms, Rate constant determination, etc.
•Molecular Biology Techniques: Cloning and Expression of Recombinant Proteins, Gene Expression in Cell Free Cellular Component System, PCR Techniques Hybridization Techniques, Co-Expression of Proteins, Dual Expression Cassette Design, DNA/RNA Synthesis Techniques.
•Biophysical studies: Fluorescence, CD, Chemical Modification of Active Site Residues, Anisotropy.
•Protein-Nucleic acid Interactions: Gel mobility shift assay, Chemical foot printing.
•Molecular Virology: Transfection, Virus titer, Virus Infection, Luciferase Assay, Real Time PCR, FACS analysis.
Memberships in Professional & Honorary Societies:
•American Society for Biochemistry and Molecular Biology (ASBMB)
•Sigma-Xi
•Center for Cancer Research Fellows and Young Investigators (CCRFYI)
•Virology Interest Group at the National Institutes of Health
Professional Experience in Leadership Positions:
Committee Member Distinguished Seminar Series at NCI-Frederick.
•Co-Chaired the Molecular and Cellular Biology Session of CCRFYI Retreat 2004 and 2005.
•Committee Member CCRFYI in 2004.
•Committee Member for the Outstanding Mentor Award at the NCI.
Advisor: Guidepoint Global Advisors
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Available Modes Of Communication
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email/chat
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Contact
CV Dash
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